4K9K

Crystal Structure of the His281Tyr mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Benzoylformate decarboxylase	polymer	530	56800.0	1	UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) In PDB	Pseudomonas putida	BFD, BFDC
2	A	THIAMINE DIPHOSPHATE	non-polymer		425.3	1	Chemie (TPP)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5		water	water		18.0	622	Chemie (HOH)

Sequence modifications

A: 2 - 525 (UniProt: P20906)

PDB	External Database	Details
Tyr 281	His 281	engineered mutation
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56800.0
Non-Polymers*	Number of molecules	5
	Total formula weight	538.3
All*	Total formula weight	57338.3

*Water molecules are not included.