4K8G
Crystal structure of D-Mannonate dehydratase from Novosphingobium aromaticivorans mutant (V161A, R163A, K165G, L166A, Y167G, Y168A, E169G)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Mandelate racemase/muconate lactonizing enzyme, N-terminal domain protein | polymer | 422 | 46970.1 | 1 | UniProt (A4XF23) Pfam (PF02746) Pfam (PF13378) In PDB | Novosphingobium aromaticivorans | |
2 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
3 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
4 | water | water | 18.0 | 534 | Chemie (HOH) |
Sequence modifications
A: 1 - 402 (UniProt: A4XF23)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 161 | Val 161 | engineered mutation |
Ala 163 | Arg 163 | engineered mutation |
Gly 165 | Lys 165 | engineered mutation |
Ala 166 | Leu 166 | engineered mutation |
Gly 167 | Tyr 167 | engineered mutation |
Ala 168 | Tyr 168 | engineered mutation |
Gly 169 | Glu 169 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 46970.1 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 116.4 | |
All* | Total formula weight | 47086.5 |