4K4U

Poliovirus polymerase elongation complex (r5_form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	RNA-directed RNA polymerase 3D-POL	polymer	471	53789.3	2	UniProt (P03300) Pfam (PF00680) In PDB	Human poliovirus 1
2	B, F	RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*UP*CP*GP*UP*CP*GP*AP*AP*A)-3')	polymer	26	8240.9	2
3	C, G	RNA (5'-R(*UP*GP*UP*UP*CP*GP*AP*CP*GP*AP*GP*AP*GP*AP*GP*A)-3')	polymer	16	5201.2	2
4	H, D	RNA (5'-R(P*GP*GP*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')	polymer	11	3665.3	2
5	A, E	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
6		water	water		18.0	57	Chemie (HOH)

Sequence modifications

A, E: 1 - 461 (UniProt: P03300)

PDB	External Database	Details
Met 290	Cys 2038	engineered mutation
Asp 446	Leu 2194	engineered mutation
Gly 462	-	expression tag
Ser 463	-	expression tag
Ser 464	-	expression tag
Ser 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	141793.2
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	142177.5

*Water molecules are not included.