4K4T

Poliovirus polymerase elongation complex (r4_form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	RNA-directed RNA polymerase 3D-POL	polymer	471	53761.2	2	UniProt (P03300) Pfam (PF00680)	Human poliovirus 1	Genome polyprotein
2	B, F (B, F)	RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*GP*AP*AP*A)-3')	polymer	22	7018.2	2
3	C, G (C, G)	RNA (5'-R(P*UP*GP*UP*UP*CP*CP*GP*AP*GP*AP*GP*A)-3')	polymer	12	3852.3	2
4	D, H (D, H)	RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')	polymer	9	2974.9	2
5	I, K, L, M (A, B, E)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
6	J, N (A, E)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	O, P, Q, R, S... (A, B, C, D, E...)	water	water		18.0	98	Chemie (HOH)

Sequence modifications

A, E: 1 - 461 (UniProt: P03300)

PDB	External Database	Details
Asp 446	Leu 2194	engineered mutation
Gly 462	-	expression tag
Ser 463	-	expression tag
Ser 464	-	expression tag
Ser 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	135213.2
Non-Polymers*	Number of molecules	6
	Total formula weight	499.2
All*	Total formula weight	135712.4

*Water molecules are not included.