4K3J

Crystal structure of Onartuzumab Fab in complex with MET and HGF-beta

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Hepatocyte growth factor	polymer	233	26038.1	1	UniProt (P14210) Pfam (PF00089)	Homo sapiens (human)	Hepatopoietin-A, Scatter factor, SF, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain
2	B (B)	Hepatocyte growth factor beta chain	polymer	534	60758.9	1	UniProt (P08581) Pfam (PF01403) Pfam (PF01437)	Homo sapiens (human)	HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met
3	C (H)	Onartuzumab Fab heavy chain	polymer	224	24000.8	1		Mus musculus Homo sapiens
4	D (L)	Onartuzumab Fab light chain	polymer	220	24363.0	1		Mus musculus Homo sapiens
5	E (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	F, G, H, I (A, B, H, L)	water	water		18.0	183	Chemie (HOH)

Sequence modifications

A: 495 - 721 (UniProt: P14210)

PDB	External Database	Details
Ser 604	Cys 604	engineered mutation
His 722	-	expression tag
His 723	-	expression tag
His 724	-	expression tag
His 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag

B: 39 - 564 (UniProt: P08581)

PDB	External Database	Details
His 565	-	expression tag
His 566	-	expression tag
His 567	-	expression tag
His 568	-	expression tag
His 569	-	expression tag
His 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135160.8
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	135382.0

*Water molecules are not included.