4K1W
Crystal structure of the A314P mutant of mannonate dehydratase from novosphingobium aromaticivorans complexed with mg and d-mannonate
Replaces: 3R4EEntity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Mandelate racemase/muconate lactonizing enzyme, N-terminal domain protein | polymer | 418 | 47132.4 | 4 | UniProt (A4XF23) Pfam (PF02746) Pfam (PF13378) In PDB | Novosphingobium aromaticivorans | |
2 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
3 | A, B, C, D | D-MANNONIC ACID | non-polymer | 196.2 | 4 | Chemie (CS2) | |||
4 | A, B, C, D | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 4 | Chemie (PGE) | |||
5 | A, C, D | CARBON DIOXIDE | non-polymer | 44.0 | 5 | Chemie (CO2) | |||
6 | D, A, B, C | 1,3-PROPANDIOL | non-polymer | 76.1 | 4 | Chemie (PDO) | |||
7 | C | CARBONATE ION | non-polymer | 60.0 | 1 | Chemie (CO3) | |||
8 | water | water | 18.0 | 1003 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 402 (UniProt: A4XF23)
PDB | External Database | Details |
---|---|---|
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Pro 314 | Ala 314 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 188529.6 | |
Non-Polymers* | Number of molecules | 23 |
Total formula weight | 2091.3 | |
All* | Total formula weight | 190620.9 |