4JZS

Crystal structure of the Bacillus subtilis pyrophosphohydrolase BsRppH (E68A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	dGTP pyrophosphohydrolase	polymer	178	20653.6	2	UniProt (O35013) Pfam (PF00293)	Bacillus subtilis subsp. subtilis	8-oxo-dGTPase, 7,8-dihydro-8-oxoguanine-triphosphatase
2	C (D)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
3	D, E (A, D)	water	water		18.0	58	Chemie (HOH)

Sequence modifications

A, D: 1 - 158 (UniProt: O35013)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 68	Glu 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	41307.2
Non-Polymers*	Number of molecules	1
	Total formula weight	238.3
All*	Total formula weight	41545.5

*Water molecules are not included.