4JWS

Crystal structure of Cytochrome P450cam-putidaredoxin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Camphor 5-monooxygenase	polymer	415	46597.7	2	UniProt (P00183) Pfam (PF00067)	Pseudomonas putida	Cytochrome P450-cam, Cytochrome P450cam
2	C, D (C, D)	Putidaredoxin	polymer	112	12257.8	2	UniProt (P00259) Pfam (PF00111)	Pseudomonas putida	PDX
3	E, H (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
4	F, I (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	G, J (A, B)	1,1'-hexane-1,6-diyldipyrrolidine-2,5-dione	non-polymer		280.3	2	Chemie (1N0)
6	K, L (C, D)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
7	M, N, O, P (A, B, C, D)	water	water		18.0	231	Chemie (HOH)

Sequence modifications

A, B: 0 - 414 (UniProt: P00183)

PDB	External Database	Details
Ser 58	Cys 59	engineered mutation
Ser 85	Cys 86	engineered mutation
Ser 136	Cys 137	engineered mutation
Ser 285	Cys 286	engineered mutation
Ala 334	Cys 335	engineered mutation
Cys 344	Lys 345	engineered mutation

C, D: 1 - 106 (UniProt: P00259)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117711.0
Non-Polymers*	Number of molecules	8
	Total formula weight	2225.4
All*	Total formula weight	119936.4

*Water molecules are not included.