4JW3

Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Neocarzinostatin	polymer	120	12196.2	2	UniProt (P0A3S0) Pfam (PF00960)	Streptomyces malayensis	NCS, Mitomalcin, MMC
2	C, D (C, D)	Alpha-helical artificial proteins	polymer	139	15493.6	2		synthetic construct (artificial)
3	E, F, G, H (A, B, C, D)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B: 1 - 112 (UniProt: P0A3S0)

PDB	External Database	Details
Trp 33	Asp 33	engineered mutation
Ala 35	Gly 35	engineered mutation
Trp 37	Cys 37	engineered mutation
Arg 39	Trp 39	engineered mutation
Trp 45	Leu 45	engineered mutation
Tyr 47	Cys 47	engineered mutation
Asn 52	Phe 52	engineered mutation
Ala 113	-	expression tag
Ala 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	55379.6
All*	Total formula weight	55379.6

*Water molecules are not included.