4JUF

Crystal Structure of His281Ala mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Benzoylformate decarboxylase	polymer	533	57033.4	4	UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Pseudomonas putida	BFD, BFDC
2	E, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	F, J, L, M, P (A, B, C, D)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
4	G, K, N, Q (A, B, C, D)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
5	H, O (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	R, S, T, U (A, B, C, D)	water	water		18.0	1029	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 528 (UniProt: P20906)

PDB	External Database	Details
Ala 281	His 281	engineered mutation
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228133.5
Non-Polymers*	Number of molecules	13
	Total formula weight	2134.4
All*	Total formula weight	230267.9

*Water molecules are not included.