4JUC

Crystal Structure of the Ser26Met mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Benzoylformate decarboxylase	polymer	534	57275.8	4	UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Pseudomonas putida	BFD, BFDC
2	E, I, M, Q (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	F, J, N, R (A, B, C, D)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
4	G, H, K, L, O... (A, B, C)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
5	S, T, U, V (A, B, C, D)	water	water		18.0	684	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 528 (UniProt: P20906)

PDB	External Database	Details
Met 26	Ser 26	engineered mutation
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	229103.0
Non-Polymers*	Number of molecules	14
	Total formula weight	2414.1
All*	Total formula weight	231517.2

*Water molecules are not included.