4JTD
Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, G | Voltage-gated potassium channel subunit beta-2 | polymer | 333 | 37353.1 | 2 | UniProt (P62483) Pfam (PF00248) In PDB | Rattus norvegicus (brown rat rat rats) | K(+) channel subunit beta-2, Kv-beta-2 |
2 | B, H | Potassium voltage-gated channel subfamily A member 2, Potassium voltage-gated channel subfamily B member 1 | polymer | 514 | 58821.7 | 2 | UniProt (P63142) UniProt (P15387) Pfam (PF02214) Pfam (PF00520) Pfam (PF07885) In PDB | Rattus norvegicus (brown rat rat rats) | RAK, RBK2, RCK5, Voltage-gated potassium channel subunit Kv1.2, Delayed rectifier potassium channel 1, DRK1, Voltage-gated potassium channel subunit Kv2.1 |
3 | Y | Potassium channel toxin alpha-KTx 1.1 | polymer | 37 | 4312.0 | 1 | UniProt (P13487) Pfam (PF00451) In PDB | Leiurus quinquestriatus hebraeus (Yellow scorpion) | ChTX-Lq1, ChTx-a, Charybdotoxin, ChTX |
4 | G, A | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
5 | H, B | POTASSIUM ION | non-polymer | 39.1 | 8 | Chemie (K) | |||
6 | H, B | (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate | non-polymer | 749.0 | 17 | Chemie (PGW) | |||
7 | water | water | 18.0 | 313 | Chemie (HOH) |
Sequence modifications
A, G: 36 - 367 (UniProt: P62483)
B, H: 1 - 266 (UniProt: P63142)
B, H: 267 - 299 (UniProt: P15387)
B, H: 300 - 495 (UniProt: P63142)
Y: 1 - 37 (UniProt: P13487)
PDB | External Database | Details |
---|---|---|
Met 35 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -18 | - | expression tag |
Ala -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
Gly -6 | - | expression tag |
Leu -5 | - | expression tag |
Val -4 | - | expression tag |
Pro -3 | - | expression tag |
Arg -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Ser 31 | Cys 31 | engineered mutation |
Ser 32 | Cys 32 | engineered mutation |
Gln 207 | Asn 207 | engineered mutation |
B, H: 300 - 495 (UniProt: P63142)
PDB | External Database | Details |
---|---|---|
Ser 431 | Cys 435 | engineered mutation |
Ser 478 | Cys 482 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 27 | Lys 49 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 196661.5 | |
Non-Polymers* | Number of molecules | 27 |
Total formula weight | 14532.7 | |
All* | Total formula weight | 211194.2 |