4JTD

Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, G)	Voltage-gated potassium channel subunit beta-2	polymer	333	37353.1	2	UniProt (P62483) Pfam (PF00248)	Rattus norvegicus (brown rat rat rats)	K(+) channel subunit beta-2, Kv-beta-2
2	B, D (B, H)	Potassium voltage-gated channel subfamily A member 2, Potassium voltage-gated channel subfamily B member 1	polymer	514	58821.7	2	UniProt (P63142) UniProt (P15387) Pfam (PF02214) Pfam (PF00520) Pfam (PF07885)	Rattus norvegicus (brown rat rat rats)	RAK, RBK2, RCK5, Voltage-gated potassium channel subunit Kv1.2, Delayed rectifier potassium channel 1, DRK1, Voltage-gated potassium channel subunit Kv2.1
3	E (Y)	Potassium channel toxin alpha-KTx 1.1	polymer	37	4312.0	1	UniProt (P13487) Pfam (PF00451)	Leiurus quinquestriatus hebraeus (Yellow scorpion)	ChTX-Lq1, ChTx-a, Charybdotoxin, ChTX
4	AA, F (G, A)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
5	BA, CA, DA, EA, G... (H, B)	POTASSIUM ION	non-polymer		39.1	8	Chemie (K)
6	FA, K, L, M, N... (H, B)	(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate	non-polymer		749.0	17	Chemie (PGW)
7	GA, HA, IA, JA (A, B, G, H)	water	water		18.0	313	Chemie (HOH)

Sequence modifications

A, G: 36 - 367 (UniProt: P62483)

PDB	External Database	Details
Met 35	-	expression tag

B, H: 1 - 266 (UniProt: P63142)

PDB	External Database	Details
Met -18	-	expression tag
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 31	Cys 31	engineered mutation
Ser 32	Cys 32	engineered mutation
Gln 207	Asn 207	engineered mutation

B, H: 267 - 299 (UniProt: P15387)
B, H: 300 - 495 (UniProt: P63142)

PDB	External Database	Details
Ser 431	Cys 435	engineered mutation
Ser 478	Cys 482	engineered mutation

Y: 1 - 37 (UniProt: P13487)

PDB	External Database	Details
Met 27	Lys 49	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	196661.5
Non-Polymers*	Number of molecules	27
	Total formula weight	14532.7
All*	Total formula weight	211194.2

*Water molecules are not included.