4JKG

Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain with bound Mg ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pre-mRNA-processing-splicing factor 8	polymer	222	25452.7	1	UniProt (Q6P2Q9) Pfam (PF12134) In PDB	Homo sapiens	220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220
2	B	Pre-mRNA-processing-splicing factor 8	polymer	222	25448.7	1	UniProt (Q6P2Q9) Pfam (PF12134) In PDB	Homo sapiens	220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220
3	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4		water	water		18.0	509	Chemie (HOH)

Sequence modifications

A: 1769 - 1990 (UniProt: Q6P2Q9)

PDB	External Database	Details
Ala 1865	Arg 1865	engineered mutation

B: 1769 - 1990 (UniProt: Q6P2Q9)

PDB	External Database	Details
Pro 1789	Thr 1789	engineered mutation
Ala 1865	Arg 1865	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50901.4
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	50925.7

*Water molecules are not included.