4JK1

X-ray crystal structure of Escherichia coli sigma70 holoenzyme in complex with Guanosine tetraphosphate (ppGpp)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H (A, B, F, G)	Escherichia coli RNA polymerase alpha subunit	polymer	329	36558.7	4	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, I (C, H)	Escherichia coli RNA polymerase beta subunit	polymer	1342	150820.9	2	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, J (D, I)	Escherichia coli RNA polymerase beta' subunit	polymer	1407	155366.8	2	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E, K (E, J)	Escherichia coli RNA polymerase omega subunit	polymer	91	10249.5	2	UniProt (H0QDQ9) Pfam (PF01192) UniProt (by SIFTS) (P0A800)	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	F, L (X, Y)	Escherichia coli RNA polymerase sigma70 subunit	polymer	613	70352.2	2	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Escherichia coli	Sigma-70
6	M, N, P, Q (D, I)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	O (D)	GUANOSINE-5',3'-TETRAPHOSPHATE	non-polymer		603.2	1	Chemie (G4P)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	919813.6
Non-Polymers*	Number of molecules	5
	Total formula weight	864.8
All*	Total formula weight	920678.4

*Water molecules are not included.