4JFG

Crystal structure of sfGFP-66-HqAla

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Green fluorescent protein	polymer	244	27680.1	8	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	E, F, A, G, B...	quinolin-8-ol	non-polymer		145.2	8	Chemie (HQY)
3	D, E, F, A, G...	CESIUM ION	non-polymer		132.9	31	Chemie (CS)
4		water	water		18.0	130	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Gly -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
Val 206	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	221440.9
Non-Polymers*	Number of molecules	39
	Total formula weight	5281.3
All*	Total formula weight	226722.2

*Water molecules are not included.