4JEY

E198A mutant of N-acetylornithine aminotransferase from Salmonella typhimurium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Acetylornithine/succinyldiaminopimelate aminotransferase	polymer	422	45680.9	2	UniProt (P40732) Pfam (PF00202)	Salmonella typhimurium	ACOAT, DapATase, Succinyldiaminopimelate transferase
2	C, M (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	D, E, G, I, L... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
4	F, H, K, R, S (A, B)	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
5	J, Q (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	U, V (A, B)	water	water		18.0	729	Chemie (HOH)

Sequence modifications

A, B: 1 - 405 (UniProt: P40732)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 198	Glu 198	engineered mutation
Thr 298	Ala 298	engineered mutation
Leu 406	-	expression tag
Glu 407	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91361.8
Non-Polymers*	Number of molecules	18
	Total formula weight	1394.1
All*	Total formula weight	92755.9

*Water molecules are not included.