4JAF
STRUCTURAL DETERMINATION OF THE A50T:S279G:S280K:V281K:K282E:H283N VARIANT OF CITRATE SYNTHASE FROM E. COLI COMPLEXED with NADH
Replaces: 3L98Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Citrate synthase | polymer | 426 | 47994.8 | 2 | UniProt (P0ABH7) Pfam (PF00285) | Escherichia coli | |
| 2 | C, D, F, G (A, B) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 3 | E, H (A, B) | 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE | non-polymer | 665.4 | 2 | Chemie (NAI) PubChem (928) PubChem (11250888) PubChem (12309341) PubChem (16219774) PubChem (44119956) PubChem (44415182) PubChem (439153) PubChem (124934832) PubChem (126455899) PubChem (134693949) PubChem (12309339) PubChem (12309347) PubChem (140875346) PubChem (162951601) PubChem (162951600) PubChem (164575491) PubChem (169545638) PubChem (170905224) PubChem (174316169) PubChem (94865921) PubChem (94865923) PubChem (124432988) PubChem (124934831) PubChem (124934833) PubChem (129607000) PubChem (129628338) PubChem (146167236) PubChem (162951602) PubChem (174084875) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 435 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 426 (UniProt: P0ABH7)
| PDB | External Database | Details |
|---|---|---|
| Asp 10 | Asn 11 | SEE REMARK 999 |
| Thr 50 | Ala 51 | engineered mutation |
| Gly 279 | Ser 280 | engineered mutation |
| Lys 280 | Ser 281 | engineered mutation |
| Lys 281 | Val 282 | engineered mutation |
| Glu 282 | Lys 283 | engineered mutation |
| Asn 283 | His 284 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95989.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1715.1 | |
| All* | Total formula weight | 97704.7 |
