4JAE
STRUCTURAL DETERMINATION OF THE A50T:S279G:S280K:V281K:K282E:H283N VARIANT OF CITRATE SYNTHASE FROM E. COLI complexed WITH S-CARBOXYMETHYL-COA
Replaces: 3L97Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Citrate synthase | polymer | 426 | 47994.8 | 2 | UniProt (P0ABH7) Pfam (PF00285) | Escherichia coli | |
| 2 | C, D, F, G (A, B) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 3 | E, H (A, B) | CARBOXYMETHYL COENZYME *A | non-polymer | 825.6 | 2 | Chemie (CMC) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 227 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 426 (UniProt: P0ABH7)
| PDB | External Database | Details |
|---|---|---|
| Asp 10 | Asn 11 | SEE REMARK 999 |
| Thr 50 | Ala 51 | engineered mutation |
| Gly 279 | Ser 280 | engineered mutation |
| Lys 280 | Ser 281 | engineered mutation |
| Lys 281 | Val 282 | engineered mutation |
| Glu 282 | Lys 283 | engineered mutation |
| Asn 283 | His 284 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95989.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2035.4 | |
| All* | Total formula weight | 98025.0 |
