4J9A

Engineered Digoxigenin binder DIG10.3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Engineered Digoxigenin binder protein DIG10.3	polymer	137	15997.1	9	UniProt (Q9HYR3) Pfam (PF12680)	Pseudomonas aeruginosa
2	J, K, L, M, N... (A, B, C, D, E...)	DIGOXIGENIN	non-polymer		390.5	9	Chemie (DOG)

Sequence modifications

A, B, C, D, E, F, G, H, I: 1 - 131 (UniProt: Q9HYR3)

PDB	External Database	Details
Val 7	Leu 7	engineered mutation
Ala 10	Ser 10	engineered mutation
Ser 23	Cys 23	engineered mutation
Tyr 34	Phe 34	engineered mutation
Pro 37	Ala 37	engineered mutation
Tyr 41	Trp 41	engineered mutation
Tyr 61	His 61	engineered mutation
Met 62	Leu 62	engineered mutation
Ile 64	Val 64	engineered mutation
Leu 90	Ala 90	engineered mutation
Ala 92	Val 92	engineered mutation
Tyr 99	Gln 99	engineered mutation
Ala 103	Ser 103	engineered mutation
Trp 105	Leu 105	engineered mutation
Leu 117	Asp 117	engineered mutation
Phe 119	Trp 119	engineered mutation
Val 124	His 124	engineered mutation
Pro 127	Ala 127	engineered mutation
Leu 130	Gly 130	engineered mutation
Glu 131	Val 131	engineered mutation
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	143974.0
Non-Polymers*	Number of molecules	9
	Total formula weight	3514.6
All*	Total formula weight	147488.6

*Water molecules are not included.