4IZW

The E41L mutant of the amidase from Nesterenkonia sp. AN1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Amidase	polymer	283	30084.8	1	UniProt (D0VWZ1) Pfam (PF00795) In PDB	Nesterenkonia sp. 10004
2	A	ACETAMIDE	non-polymer		59.1	1	Chemie (ACM)
3	A	2-fluoroacetamide	non-polymer		77.1	3	Chemie (FTM)
4		water	water		18.0	162	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: D0VWZ1)

PDB	External Database	Details
Met -19	-	INITIATING METHIONINE
Gly -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
Ser -16	-	EXPRESSION TAG
His -15	-	EXPRESSION TAG
His -14	-	EXPRESSION TAG
His -13	-	EXPRESSION TAG
His -12	-	EXPRESSION TAG
His -11	-	EXPRESSION TAG
His -10	-	EXPRESSION TAG
Ser -9	-	EXPRESSION TAG
Ser -8	-	EXPRESSION TAG
Gly -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Val -5	-	EXPRESSION TAG
Pro -4	-	EXPRESSION TAG
Arg -3	-	EXPRESSION TAG
Gly -2	-	EXPRESSION TAG
Ser -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
Leu 41	Glu 41	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30084.8
Non-Polymers*	Number of molecules	4
	Total formula weight	290.2
All*	Total formula weight	30375.0

*Water molecules are not included.