4IZV

The E41Q/C145A double mutant of the amidase from Nesterenkonia sp. AN1 in complex with acrylamide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Amidase	polymer	283	30067.7	1	UniProt (D0VWZ1) Pfam (PF00795) In PDB	Nesterenkonia sp. 10004
2	A	PROPIONAMIDE	non-polymer		73.1	1	Chemie (ROP)
3	A	prop-2-enamide	non-polymer		71.1	2	Chemie (1HC)
4		water	water		18.0	252	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: D0VWZ1)

PDB	External Database	Details
Met -19	-	INITIATING METHIONINE
Gly -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
Ser -16	-	EXPRESSION TAG
His -15	-	EXPRESSION TAG
His -14	-	EXPRESSION TAG
His -13	-	EXPRESSION TAG
His -12	-	EXPRESSION TAG
His -11	-	EXPRESSION TAG
His -10	-	EXPRESSION TAG
Ser -9	-	EXPRESSION TAG
Ser -8	-	EXPRESSION TAG
Gly -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Val -5	-	EXPRESSION TAG
Pro -4	-	EXPRESSION TAG
Arg -3	-	EXPRESSION TAG
Gly -2	-	EXPRESSION TAG
Ser -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
Gln 41	Glu 41	ENGINEERED MUTATION
Ala 145	Cys 145	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30067.7
Non-Polymers*	Number of molecules	3
	Total formula weight	215.3
All*	Total formula weight	30283.0

*Water molecules are not included.