4IZS

The C145A mutant of the amidase from Nesterenkonia sp. AN1 in complex with butyramide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Amidase	polymer	283	30068.7	1	UniProt (D0VWZ1) Pfam (PF00795) In PDB	Nesterenkonia sp. 10004
2	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
3	A	BUTYRAMIDE	non-polymer		87.1	1	Chemie (BMD)
4		water	water		18.0	230	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: D0VWZ1)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 145	Cys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30068.7
Non-Polymers*	Number of molecules	4
	Total formula weight	405.5
All*	Total formula weight	30474.2

*Water molecules are not included.