4IZM

Crystal structure of GltPh L66C-S300C mutant crosslinked with divalent mercury

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	425aa long hypothetical proton glutamate symport protein	polymer	421	44458.7	3	UniProt (O59010) Pfam (PF00375) In PDB	Pyrococcus horikoshii
2	A, B, C	SODIUM ION	non-polymer		23.0	6	Chemie (NA)
3	A, B, C	ASPARTIC ACID	non-polymer		133.1	3	Chemie (ASP)
4	A, B, C	MERCURY (II) ION	non-polymer		200.6	3	Chemie (HG)

Sequence modifications

A, B, C: 1 - 417 (UniProt: O59010)

PDB	External Database	Details
His 37	Asp 37	engineered mutation
His 40	Lys 40	engineered mutation
Cys 66	Leu 66	engineered mutation
His 125	Lys 125	engineered mutation
His 132	Lys 132	engineered mutation
His 223	Lys 223	engineered mutation
His 264	Lys 264	engineered mutation
Cys 300	Ser 300	engineered mutation
Ala 321	Cys 321	engineered mutation
His 368	Glu 368	engineered mutation
Thr 418	-	expression tag
Leu 419	-	expression tag
Val 420	-	expression tag
Pro 421	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	133376.2
Non-Polymers*	Number of molecules	12
	Total formula weight	1139.0
All*	Total formula weight	134515.2

*Water molecules are not included.