4IT1

Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative glucarate dehydratase	polymer	446	49485.0	4	UniProt (Q3KB33) Pfam (PF02746) Pfam (PF13378)	Pseudomonas fluorescens
2	E, K (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	F, G, I, L (A, B, C, D)	L(+)-TARTARIC ACID	non-polymer		150.1	4	Chemie (TLA)
4	H, J (B, C)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	M (D)	BICARBONATE ION	non-polymer		61.0	1	Chemie (BCT)
6	N, O, P, Q (A, B, C, D)	water	water		18.0	633	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 424 (UniProt: Q3KB33)

PDB	External Database	Details
Met -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	197940.2
Non-Polymers*	Number of molecules	9
	Total formula weight	788.2
All*	Total formula weight	198728.3

*Water molecules are not included.