4IST

S177A Kluyveromyces lactis Allophanate Hydrolase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Allophanate Hydrolase	polymer	644	70589.4	2	UniProt (Q6CP22) Pfam (PF01425) Pfam (PF21986) In PDB	Kluyveromyces lactis (Yeast)	KLLA0E08119p
2	A, B	D(-)-TARTARIC ACID	non-polymer		150.1	2	Chemie (TAR)
3		water	water		18.0	138	Chemie (HOH)

Sequence modifications

A, B: 1 - 621 (UniProt: Q6CP22)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 177	Ser 177	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	141178.8
Non-Polymers*	Number of molecules	2
	Total formula weight	300.2
All*	Total formula weight	141478.9

*Water molecules are not included.