4IQZ

The crystal structure of a large insert in RNA polymerase (RpoC) subunit from E. coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	DNA-directed RNA polymerase subunit beta'	polymer	250	26734.1	5	UniProt (P0A8T7) Pfam (PF04998)	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
2	AA, AB, BA, BB, CA... (B, D, A, C, E)	IODIDE ION	non-polymer		126.9	56	Chemie (IOD)
3	JA, KB, PB, U, V... (B, D, E, A, C)	SODIUM ION	non-polymer		23.0	7	Chemie (NA)
4	QB, RB, SB, TB, UB (A, B, C, D, E)	water	water		18.0	528	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 21 - 230 (UniProt: P0A8T7)

PDB	External Database	Details
Mse 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Mse 230	Val 1141	engineered mutation
Asp 231	-	expression tag
Pro 232	-	expression tag
Ala 233	-	expression tag
Ala 234	-	expression tag
Asn 235	-	expression tag
Lys 236	-	expression tag
Ala 237	-	expression tag
Arg 238	-	expression tag
Lys 239	-	expression tag
Glu 240	-	expression tag
Ala 241	-	expression tag
Glu 242	-	expression tag
Leu 243	-	expression tag
Ala 244	-	expression tag
Ala 245	-	expression tag
Ala 246	-	expression tag
Thr 247	-	expression tag
Ala 248	-	expression tag
Glu 249	-	expression tag
Gln 250	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	133670.6
Non-Polymers*	Number of molecules	63
	Total formula weight	7267.6
All*	Total formula weight	140938.1

*Water molecules are not included.