4INZ

The crystal structure of M145A mutant of an epoxide hydrolase from Bacillus megaterium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Soluble epoxide hydrolase	polymer	304	35226.6	2	UniProt (G9BEX6) Pfam (PF00561) In PDB	Bacillus megaterium
2	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4		water	water		18.0	394	Chemie (HOH)

Sequence modifications

A, B: 1 - 287 (UniProt: G9BEX6)

PDB	External Database	Details
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 145	Met 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70453.1
Non-Polymers*	Number of molecules	3
	Total formula weight	274.3
All*	Total formula weight	70727.4

*Water molecules are not included.