4INE

Crystal structure of N-methyl transferase (PMT-2) from Caenorhabditis elegant complexed with S-adenosyl homocysteine and phosphoethanolamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Protein PMT-2	polymer	454	51711.0	2	UniProt (Q22993) Pfam (PF17987) Pfam (PF08241) In PDB	Caenorhabditis elegans (nematode)
2	A, B	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3	A, B	PHOSPHORIC ACID MONO-(2-AMINO-ETHYL) ESTER	non-polymer		141.1	2	Chemie (OPE)
4	A, B	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
5		water	water		18.0	1086	Chemie (HOH)

Sequence modifications

A, B: 1 - 437 (UniProt: Q22993)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
-	Asn 90	SEE REMARK 999
-	Ala 91	SEE REMARK 999
-	Thr 92	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	103421.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1207.2
All*	Total formula weight	104629.1

*Water molecules are not included.