4IMI

Novel Modifications on C-terminal Domain of RNA Polymerase II can Fine- tune the Phosphatase Activity of Ssu72.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Symplekin	polymer	339	37495.3	2	UniProt (Q8MSU4) Pfam (PF11935)	Drosophila melanogaster (Fruit fly)	LD45768p
2	B, D (B, D)	CG14216	polymer	200	23130.3	2	UniProt (Q9VWE4) Pfam (PF04722)	Drosophila melanogaster (Fruit fly)	LD40846p
3	E (F)	CTD	polymer	19	2139.0	1		Synthetic
4	F (B)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	G, H, I, J, K (A, B, C, D, F)	water	water		18.0	199	Chemie (HOH)

Sequence modifications

A, C: 19 - 351 (UniProt: Q8MSU4)

PDB	External Database	Details
Gly 13	-	expression tag
Pro 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Gly 17	-	expression tag
Met 18	-	expression tag

B, D: 1 - 195 (UniProt: Q9VWE4)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asp 13	Cys 13	engineered mutation
Asn 144	Asp 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	123390.1
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	123485.1

*Water molecules are not included.