4IHJ

Crystal structure of tubulin-stathmin-TTL-ADP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Tubulin alpha-1B chain	polymer	450	50041.3	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (bovine cow domestic cattle domestic cow)	Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain
2	B, D	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (bovine cow domestic cattle domestic cow)
3	E	Stathmin-4	polymer	143	16844.2	1	UniProt (P63043) Pfam (PF00836) In PDB	Rattus norvegicus (brown rat rat rats)	Stathmin-like protein B3, RB3
4	F	TUBULIN TYROSINE LIGASE, TTL	polymer	384	44378.5	1	UniProt (E1BQ43) In PDB	Gallus gallus (bantam chickens)
5	A, C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	A, B, C, D, F	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
7	A, B, C	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	B, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
10	B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
11	F	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
12	F	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
13		water	water		18.0	1033	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	cloning artifact
Ala 4	-	cloning artifact

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261305.0
Non-Polymers*	Number of molecules	20
	Total formula weight	3208.4
All*	Total formula weight	264513.4

*Water molecules are not included.