4IG6

Crystal structure of a tRNA (guanine-N1)-methyltransferase from Anaplasma phagocytophilum bound to S-adenosylhomocysteine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (guanine-N(1)-)-methyltransferase	polymer	253	28076.0	1	UniProt (Q2GIL5) Pfam (PF01746)	Anaplasma phagocytophilum	M1G-methyltransferase, tRNA [GM37] methyltransferase
2	B (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	C, D (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	E (A)	water	water		18.0	141	Chemie (HOH)

Sequence modifications

A: 1 - 232 (UniProt: Q2GIL5)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Thr -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Ala -7	-	expression tag
Gln -6	-	expression tag
Thr -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28076.0
Non-Polymers*	Number of molecules	3
	Total formula weight	455.3
All*	Total formula weight	28531.3

*Water molecules are not included.