4IBX
Crystal structure of stabilized TEM-1 beta-lactamase variant v.13
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Beta-lactamase TEM | polymer | 263 | 28770.8 | 5 | UniProt (P62593) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2 |
| 2 | F, I (A, C) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | G, H, K (A, B, D) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 3 | Chemie (MES) | |||
| 4 | J (C) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | L, M, N, O, P (A, B, C, D, E) | water | water | 18.0 | 96 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 26 - 290 (UniProt: P62593)
| PDB | External Database | Details |
|---|---|---|
| Gly 42 | Ala 40 | engineered mutation |
| Ala 52 | Asn 50 | engineered mutation |
| Gly 120 | Arg 118 | engineered mutation |
| Thr 182 | Met 180 | engineered mutation |
| Val 184 | Ala 182 | conflict |
| Ala 201 | Leu 199 | engineered mutation |
| Met 265 | Thr 261 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 143853.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 761.9 | |
| All* | Total formula weight | 144615.8 |
