4IAP

Crystal structure of PH domain of Osh3 from Saccharomyces cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Oxysterol-binding protein homolog 3,Endolysin,Oxysterol-binding protein homolog 3	polymer	260	29746.0	2	UniProt (P38713) UniProt (P00720) Pfam (PF00959) Pfam (PF15409) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Lysis protein,Lysozyme,Muramidase
2	A, B	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
3		water	water		18.0	103	Chemie (HOH)

Sequence modifications

A, B: 221 - 233 (UniProt: P38713)

PDB	External Database	Details
Gly 217	-	expression tag
Ser 218	-	expression tag
Ala 219	-	expression tag
Met 220	-	expression tag

A, B: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	see sequence details
Asn 1020	Asp 20	engineered mutation
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	see sequence details
Val 1162	-	linker
Asp 1163	-	linker

A, B: 1237 - 1315 (UniProt: P38713)

PDB	External Database	Details
Val 1000	-	linker
Asp 1001	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59492.1
Non-Polymers*	Number of molecules	8
	Total formula weight	768.5
All*	Total formula weight	60260.6

*Water molecules are not included.