4I9Q
Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA polymerase | polymer | 903 | 104519.0 | 2 | UniProt (Q38087) Pfam (PF03104) Pfam (PF00136) In PDB | Enterobacteria phage RB69 | Gp43 |
2 | T, D | DNA (5'-D(*TP*CP*AP*CP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3') | polymer | 18 | 5501.6 | 2 | Synthetic construct | ||
3 | P, C | DNA (5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3') | polymer | 13 | 3967.6 | 2 | Synthetic construct | ||
4 | A, B | 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine | non-polymer | 506.2 | 4 | Chemie (XG4) | |||
5 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
6 | A, B | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
7 | water | water | 18.0 | 398 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 903 (UniProt: Q38087)
PDB | External Database | Details |
---|---|---|
Ala 222 | Asp 222 | engineered mutation |
Ala 327 | Asp 327 | engineered mutation |
Ala 567 | Tyr 567 | engineered mutation |
Ala 714 | Asp 714 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 227976.4 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 2150.9 | |
All* | Total formula weight | 230127.3 |