4I90
Structure of the N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from S. aureus bound to choline
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | 1-phosphatidylinositol phosphodiesterase | polymer | 303 | 34358.3 | 1 | UniProt (P45723) Pfam (PF00388) In PDB | Staphylococcus aureus subsp. aureus | Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC |
| 2 | A | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 3 | A | CHOLINE ION | non-polymer | 104.2 | 2 | Chemie (CHT) | |||
| 4 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | water | water | 18.0 | 227 | Chemie (HOH) |
Sequence modifications
A: 1 - 302 (UniProt: P45723)
| PDB | External Database | Details |
|---|---|---|
| Tyr 254 | Asn 264 | engineered mutation |
| Tyr 258 | His 268 | engineered mutation |
| Leu 303 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 34358.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 361.9 | |
| All* | Total formula weight | 34720.1 |
