4I5U

Crystal structure of a fungal chimeric cellobiohydrolase Cel6A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Chimeric cel6A	polymer	364	39740.1	1	UniProt (Q9C1S9) UniProt (P07987) UniProt (Q5G2D4) Pfam (PF01341)	Humicola insolens (Trichoderma reesei Trichoderma reesei)	1,4-beta-cellobiohydrolase 6A, Avicelase 2, Beta-glucancellobiohydrolase 6A, Exocellobiohydrolase 6A, 4-beta-cellobiohydrolase, Exocellobiohydrolase II, CBHII, Exoglucanase II
2	B, C (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
3	D, E, F, G, H... (A)	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
4	N (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
5	O, P (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
6	Q (A)	water	water		18.0	327	Chemie (HOH)

Sequence modifications

A: 90 - 134 (UniProt: Q9C1S9)

PDB	External Database	Details
Ala 128	Val 155	engineered mutation

A: 135 - 308 (UniProt: P07987)

PDB	External Database	Details
Leu 135	Met 158	engineered mutation
Leu 277	Gln 300	engineered mutation

A: 309 - 400 (UniProt: Q5G2D4)

PDB	External Database	Details
Pro 317	Ser 347	engineered mutation

A: 401 - 447 (UniProt: P07987)

PDB	External Database	Details
Pro 406	Ser 430	engineered mutation
Pro 413	Ser 437	engineered mutation
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39740.1
Non-Polymers*	Number of molecules	15
	Total formula weight	1233.3
All*	Total formula weight	40973.4

*Water molecules are not included.