4I43

Crystal structure of Prp8:Aar2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	A1 cistron-splicing factor AAR2	polymer	384	44967.1	1	UniProt (P32357) Pfam (PF20981) Pfam (PF05282)	Saccharomyces cerevisiae (yeast)
2	B (B)	Pre-mRNA-splicing factor 8	polymer	1564	180217.6	1	UniProt (P33334) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084)	Saccharomyces cerevisiae (yeast)
3	C, D (A, B)	water	water		18.0	670	Chemie (HOH)

Sequence modifications

A: 1 - 355 (UniProt: P32357)

PDB	External Database	Details
Ala 356	-	expression tag
Ala 357	-	expression tag
Gly 358	-	expression tag
Lys 359	-	expression tag
Val 360	-	expression tag
Ala 361	-	expression tag
Arg 362	-	expression tag
Gly 363	-	expression tag
Ser 364	-	expression tag
Arg 365	-	expression tag
Ser 366	-	expression tag
Gly 367	-	expression tag
Ser 368	-	expression tag
Glu 369	-	expression tag
Asn 370	-	expression tag
Leu 371	-	expression tag
Tyr 372	-	expression tag
Phe 373	-	expression tag
Gln 374	-	expression tag
Gly 375	-	expression tag
Ser 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

B: 885 - 2413 (UniProt: P33334)

PDB	External Database	Details
Met 850	-	expression tag
Ala 851	-	expression tag
Arg 852	-	expression tag
Ala 853	-	expression tag
Lys 854	-	expression tag
Arg 855	-	expression tag
Arg 856	-	expression tag
Trp 857	-	expression tag
Lys 858	-	expression tag
Lys 859	-	expression tag
Asn 860	-	expression tag
Phe 861	-	expression tag
Ile 862	-	expression tag
Ala 863	-	expression tag
Val 864	-	expression tag
Ser 865	-	expression tag
Ala 866	-	expression tag
Ala 867	-	expression tag
Asn 868	-	expression tag
Arg 869	-	expression tag
Phe 870	-	expression tag
Lys 871	-	expression tag
Lys 872	-	expression tag
Ile 873	-	expression tag
Ser 874	-	expression tag
Ser 875	-	expression tag
Ser 876	-	expression tag
Gly 877	-	expression tag
Ala 878	-	expression tag
Leu 879	-	expression tag
Gly 880	-	expression tag
Ser 881	-	expression tag
Gly 882	-	expression tag
Ser 883	-	expression tag
Gly 884	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	225184.8
All*	Total formula weight	225184.8

*Water molecules are not included.