4I0X

Crystal structure of the Mycobacterum abscessus EsxEF (Mab_3112-Mab_3113) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	ESAT-6-like protein MAB_3112	polymer	94	9796.5	6	UniProt (B1MD68) Pfam (PF06013) In PDB	Mycobacterium abscessus
2	B, D, F, H, J...	ESAT-6-like protein MAB_3113	polymer	103	11427.5	6	UniProt (B1MD69) Pfam (PF06013) In PDB	Mycobacterium abscessus
3	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	B	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
5	C	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	337	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K: 1 - 93 (UniProt: B1MD68)

PDB	External Database	Details
Ser 0	-	expression tag
Asn 23	Ser 35	engineered mutation
Lys 37	Arg 49	engineered mutation
Ala 75	Val 87	engineered mutation
Asp 93	Gly 105	engineered mutation

B, D, F, H, J, L: 1 - 103 (UniProt: B1MD69)

PDB	External Database	Details
Ala 2	Pro 2	engineered mutation
Ala 51	Thr 51	engineered mutation
Gln 60	Glu 60	engineered mutation
Ser 77	Asn 77	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	127344.3
Non-Polymers*	Number of molecules	3
	Total formula weight	266.3
All*	Total formula weight	127610.6

*Water molecules are not included.