4HS9

Methanol tolerant mutant of the Proteus mirabilis lipase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lipase	polymer	307	33913.1	1	UniProt (B4EVM3) Pfam (PF00561)	Proteus mirabilis
2	B (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	E (A)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
6	F (A)	2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL	non-polymer		354.4	1	Chemie (PE4)
7	G (A)	water	water		18.0	117	Chemie (HOH)

Sequence modifications

A: 1 - 287 (UniProt: B4EVM3)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 17	Asn 17	engineered mutation
Thr 33	Arg 33	engineered mutation
Ile 64	Leu 64	engineered mutation
Thr 70	Ala 70	engineered mutation
Ile 119	Met 119	engineered mutation
Cys 181	Gly 181	engineered mutation
Glu 202	Gly 202	engineered mutation
Asn 208	Lys 208	engineered mutation
Leu 225	Phe 225	engineered mutation
Cys 238	Ser 238	engineered mutation
Phe 255	Ile 255	engineered mutation
Ser 266	Gly 266	engineered mutation
Asn 270	Asp 270	engineered mutation
Leu 277	Gln 277	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33913.1
Non-Polymers*	Number of molecules	5
	Total formula weight	760.3
All*	Total formula weight	34673.4

*Water molecules are not included.