4HRN

Structural Basis for Eliciting a Cytotoxic Effect in HER2-Overexpressing Cancer Cells via Binding to the Extracellular Domain of HER2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Designed Ankyrin Repeat Protein H10-2-G	polymer	136	14618.4	2		synthetic
2	C, D	Receptor tyrosine-protein kinase erbB-2	polymer	103	11658.2	2	UniProt (P04626) Pfam (PF14843) In PDB	Homo sapiens (human)	Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2

Sequence modifications

C, D: 7 - 103 (UniProt: P04626)

PDB	External Database	Details
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Asp 8	Asn 530	engineered mutation
Asp 49	Asn 571	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52553.2
All*	Total formula weight	52553.2

*Water molecules are not included.