4HQV
tRNA-guanine transglycosylase Y106F, C158V, V233G mutant in complex with queuine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Queuine tRNA-ribosyltransferase | polymer | 385 | 42732.4 | 1 | UniProt (P28720) Pfam (PF01702) | Zymomonas mobilis subsp. mobilis | Guanine insertion enzyme, tRNA-guanine transglycosylase |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D, E, F (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | G (A) | 2-amino-5-({[(1S,4S,5R)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one | non-polymer | 277.3 | 1 | Chemie (QEI) | |||
| 5 | H (A) | water | water | 18.0 | 397 | Chemie (HOH) |
Sequence modifications
A: 2 - 386 (UniProt: P28720)
| PDB | External Database | Details |
|---|---|---|
| Phe 106 | Tyr 106 | engineered mutation |
| Val 158 | Cys 158 | engineered mutation |
| Gly 233 | Val 233 | engineered mutation |
| Lys 312 | Thr 312 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42732.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 711.1 | |
| All* | Total formula weight | 43443.5 |
