4HPW
Crystal structure of Tyrosine-tRNA ligase mutant complexed with unnatural amino acid 3-o-methyl-Tyrosine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tyrosine--tRNA ligase | polymer | 310 | 35293.9 | 1 | UniProt (Q57834) Pfam (PF00579) | Methanocaldococcus jannaschii | Tyrosyl-tRNA synthetase, TyrRS |
| 2 | B (A) | 3-methoxy-L-tyrosine | non-polymer | 211.2 | 1 | Chemie (3YM) | |||
| 3 | C (A) | water | water | 18.0 | 96 | Chemie (HOH) |
Sequence modifications
A: 1 - 306 (UniProt: Q57834)
| PDB | External Database | Details |
|---|---|---|
| Glu 32 | Tyr 32 | engineered mutation |
| Ser 65 | Leu 65 | engineered mutation |
| Gly 70 | His 70 | engineered mutation |
| Gly 109 | Gln 109 | engineered mutation |
| Asn 158 | Asp 158 | engineered mutation |
| Val 162 | Leu 162 | engineered mutation |
| Ala 307 | - | expression tag |
| Ala 308 | - | expression tag |
| His 309 | - | expression tag |
| His 310 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 35293.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 211.2 | |
| All* | Total formula weight | 35505.2 |
