4HNU
crystal structure of K442E mutant of S. aureus Pyruvate carboxylase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Pyruvate carboxylase | polymer | 1173 | 131168.1 | 4 | UniProt (Q99UY8) Pfam (PF00289) Pfam (PF02786) Pfam (PF02785) Pfam (PF00682) Pfam (PF02436) Pfam (PF00364) UniProt (by SIFTS) (A0A0H3JRU9) | Staphylococcus aureus | |
| 2 | E (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 3 | F, G, H, J (A, B, C, D) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 4 | I (D) | 5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL | non-polymer | 228.3 | 1 | Chemie (BTI) |
Sequence modifications
A, B, C, D: 34 - 1182 (UniProt: Q99UY8)
| PDB | External Database | Details |
|---|---|---|
| Met 11 | - | expression tag |
| Gly 12 | - | expression tag |
| Ser 13 | - | expression tag |
| Ser 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| Ser 21 | - | expression tag |
| Ser 22 | - | expression tag |
| Gly 23 | - | expression tag |
| Leu 24 | - | expression tag |
| Val 25 | - | expression tag |
| Pro 26 | - | expression tag |
| Arg 27 | - | expression tag |
| Gly 28 | - | expression tag |
| Ser 29 | - | expression tag |
| His 30 | - | expression tag |
| Met 31 | - | expression tag |
| Ala 32 | - | expression tag |
| Ser 33 | - | expression tag |
| Glu 442 | Lys 411 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 524672.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 875.3 | |
| All* | Total formula weight | 525547.6 |
