4HMY
Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | AP-1 complex subunit gamma-1 | polymer | 601 | 68194.1 | 1 | UniProt (P22892) Pfam (PF01602) | Mus musculus (mouse) | Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma-adaptin, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
| 2 | B (B) | AP-1 complex subunit beta-1 | polymer | 586 | 66186.6 | 1 | UniProt (Q10567) Pfam (PF01602) | Homo sapiens (human) | Adapter-related protein complex 1 subunit beta-1, Adaptor protein complex AP-1 subunit beta-1, Beta-1-adaptin, Beta-adaptin 1, Clathrin assembly protein complex 1 beta large chain, Golgi adaptor HA1/AP1 adaptin beta subunit |
| 3 | C (M) | AP-1 complex subunit mu-1 | polymer | 423 | 48606.7 | 1 | UniProt (P35585) Pfam (PF01217) Pfam (PF00928) | Mus musculus (mouse) | AP-mu chain family member mu1A, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, Clathrin assembly protein complex 1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin |
| 4 | D (S) | AP-1 complex subunit sigma-3 | polymer | 154 | 18321.3 | 1 | UniProt (Q96PC3) Pfam (PF01217) | Homo sapiens (human) | Adapter-related protein complex 1 sigma-1C subunit, Adaptor protein complex AP-1 sigma-1C subunit, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Sigma 1C subunit of AP-1 clathrin, Sigma-adaptin 1C, Sigma1C-adaptin |
| 5 | E (C) | ADP-ribosylation factor 1 | polymer | 172 | 19896.7 | 1 | UniProt (P84077) Pfam (PF00025) | Homo sapiens (human) | |
| 6 | F (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 7 | G (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) |
Sequence modifications
A: 1 - 595 (UniProt: P22892)
B: 1 - 584 (UniProt: Q10567)
S: 1 - 154 (UniProt: Q96PC3)
C: 17 - 181 (UniProt: P84077)
| PDB | External Database | Details |
|---|---|---|
| Glu 596 | - | expression tag |
| Asn 597 | - | expression tag |
| Leu 598 | - | expression tag |
| Tyr 599 | - | expression tag |
| Phe 600 | - | expression tag |
| Gln 601 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Arg 359 | Lys 359 | variant |
| Lys 476 | Glu 476 | variant |
| Phe 488 | Ile 488 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 148 | Ser 148 | variant |
| PDB | External Database | Details |
|---|---|---|
| Met 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 221205.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 221752.9 |
