4HMY
Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | AP-1 complex subunit gamma-1 | polymer | 601 | 68194.1 | 1 | UniProt (P22892) Pfam (PF01602) | Mus musculus (mouse) | Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma-adaptin, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
| 2 | B (B) | AP-1 complex subunit beta-1 | polymer | 586 | 66186.6 | 1 | UniProt (Q10567) Pfam (PF01602) | Homo sapiens (human) | Adapter-related protein complex 1 subunit beta-1, Adaptor protein complex AP-1 subunit beta-1, Beta-1-adaptin, Beta-adaptin 1, Clathrin assembly protein complex 1 beta large chain, Golgi adaptor HA1/AP1 adaptin beta subunit |
| 3 | C (M) | AP-1 complex subunit mu-1 | polymer | 423 | 48606.7 | 1 | UniProt (P35585) Pfam (PF01217) Pfam (PF00928) | Mus musculus (mouse) | AP-mu chain family member mu1A, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, Clathrin assembly protein complex 1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin |
| 4 | D (S) | AP-1 complex subunit sigma-3 | polymer | 154 | 18321.3 | 1 | UniProt (Q96PC3) Pfam (PF01217) | Homo sapiens (human) | Adapter-related protein complex 1 sigma-1C subunit, Adaptor protein complex AP-1 sigma-1C subunit, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Sigma 1C subunit of AP-1 clathrin, Sigma-adaptin 1C, Sigma1C-adaptin |
| 5 | E (C) | ADP-ribosylation factor 1 | polymer | 172 | 19896.7 | 1 | UniProt (P84077) Pfam (PF00025) | Homo sapiens (human) | |
| 6 | F (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 7 | G (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 595 (UniProt: P22892)
B: 1 - 584 (UniProt: Q10567)
S: 1 - 154 (UniProt: Q96PC3)
C: 17 - 181 (UniProt: P84077)
| PDB | External Database | Details |
|---|---|---|
| Glu 596 | - | expression tag |
| Asn 597 | - | expression tag |
| Leu 598 | - | expression tag |
| Tyr 599 | - | expression tag |
| Phe 600 | - | expression tag |
| Gln 601 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Arg 359 | Lys 359 | variant |
| Lys 476 | Glu 476 | variant |
| Phe 488 | Ile 488 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 148 | Ser 148 | variant |
| PDB | External Database | Details |
|---|---|---|
| Met 10 | - | expression tag |
| His 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 221205.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 221752.9 |
