4HMR

Crystal structure of mutant rabbit PRP 121-230 (S170N/S174N)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Major prion protein	polymer	132	15294.0	2	UniProt (Q95211) Pfam (PF00377)	Oryctolagus cuniculus (European rabbit Japanese white rabbit domestic rabbit rabbits)	PrP, PrP27-30, PrP33-35C
2	C, E, I (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
3	D, F (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	G, H (B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	J, K (A, B)	water	water		18.0	124	Chemie (HOH)

Sequence modifications

A, B: 120 - 230 (UniProt: Q95211)

PDB	External Database	Details
Met 99	-	expression tag
Gly 100	-	expression tag
Ser 101	-	expression tag
Ser 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
His 106	-	expression tag
His 107	-	expression tag
His 108	-	expression tag
Ser 109	-	expression tag
Ser 110	-	expression tag
Gly 111	-	expression tag
Leu 112	-	expression tag
Val 113	-	expression tag
Pro 114	-	expression tag
Arg 115	-	expression tag
Gly 116	-	expression tag
Ser 117	-	expression tag
His 118	-	expression tag
Met 119	-	expression tag
Asn 170	Ser 169	engineered mutation
Asn 174	Ser 173	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	30588.0
Non-Polymers*	Number of molecules	7
	Total formula weight	336.5
All*	Total formula weight	30924.5

*Water molecules are not included.