4HK4

Crystal structure of apo Tyrosine-tRNA ligase mutant protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tyrosine--tRNA ligase	polymer	312	35637.3	1	UniProt (Q57834) Pfam (PF00579)	Methanocaldococcus jannaschii	Tyrosyl-tRNA synthetase, TyrRS
2	B, C (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
3	D (A)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A: 1 - 306 (UniProt: Q57834)

PDB	External Database	Details
Glu 32	Tyr 32	engineered mutation
Ser 65	Leu 65	engineered mutation
Gly 70	His 70	engineered mutation
Gly 109	Gln 109	engineered mutation
Asn 158	Asp 158	engineered mutation
Val 162	Leu 162	engineered mutation
Ala 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	35637.3
Non-Polymers*	Number of molecules	2
	Total formula weight	212.2
All*	Total formula weight	35849.5

*Water molecules are not included.