4HJY

2.4 A Crystal structure of E. coli MltE-E64Q with bound chitopentaose

Replaces:  3T1Z

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Endo-type membrane-bound lytic murein transglycosylase A	polymer	206	22806.8	2	UniProt (P0C960) Pfam (PF01464) In PDB	Escherichia coli	Peptidoglycan lytic endotransglycosylase
2	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1034.0	1	In PDB GlyTouCan (G07670VS)
3	D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		627.6	1	In PDB BIRD (PRD_900017) GlyTouCan (G47362BJ)		triacetyl-beta-chitotriose
4		water	water		18.0	73	Chemie (HOH)

Sequence modifications

A, B: 17 - 203 (UniProt: P0C960)

PDB	External Database	Details
Met -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Gly 9	-	expression tag
Glu 10	-	expression tag
Asn 11	-	expression tag
Leu 12	-	expression tag
Tyr 13	-	expression tag
Phe 14	-	expression tag
Gln 15	-	expression tag
Gly 16	-	expression tag
Gln 64	Glu 64	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45613.5
Branched	Number of molecules	2
	Total formula weight	1661.6
All*	Total formula weight	47275.1

*Water molecules are not included.