4HGJ
Crystal structure of P450 BM3 5F5 heme domain variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bifunctional P-450/NADPH-P450 reductase | polymer | 455 | 52063.3 | 2 | UniProt (P14779) Pfam (PF00067) | Bacillus megaterium | Cytochrome P450(BM-3), Cytochrome P450BM-3, Cytochrome P450 102, NADPH--cytochrome P450 reductase |
| 2 | C, I (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 3 | D, E, G, H, J (A, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 4 | F (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 5 | K, L (A, B) | water | water | 18.0 | 803 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 455 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Ala 87 | Phe 88 | engineered mutation |
| Ala 235 | Thr 236 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 104126.7 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1888.7 | |
| All* | Total formula weight | 106015.4 |
