4HCN

Crystal structure of Burkholderia pseudomallei effector protein CHBP in complex with ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative ATP/GTP binding protein	polymer	255	28557.4	1	UniProt (Q63KH5) Pfam (PF16374)	Burkholderia pseudomallei	CHBP
2	B (B)	Polyubiquitin	polymer	98	11097.5	1	UniProt (P0CG63) Pfam (PF00240)	Saccharomyces cerevisiae (Baker's yeast)	Ubiquitin
3	C (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	D, F (A, B)	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
5	E (B)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	G, H (A, B)	water	water		18.0	71	Chemie (HOH)

Sequence modifications

A: 78 - 328 (UniProt: Q63KH5)

PDB	External Database	Details
Ser 74	-	expression tag
Gly 75	-	expression tag
Arg 76	-	expression tag
Pro 77	-	expression tag
Ala 156	Cys 156	engineered mutation

B: 1 - 76 (UniProt: P0CG63)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39654.9
Non-Polymers*	Number of molecules	4
	Total formula weight	293.1
All*	Total formula weight	39948.0

*Water molecules are not included.