4HB3

Crystal structure of CRM1(T539S)-Ran-RanBP1 with weakly bound unmodeled Leptomycin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GTP-binding nuclear protein Ran	polymer	216	24456.1	1	UniProt (P62826) Pfam (PF00071) In PDB	Homo sapiens (human)	Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein
2	B	Ran-specific GTPase-activating protein 1	polymer	140	16378.8	1	UniProt (P41920) Pfam (PF00638) In PDB	Saccharomyces cerevisiae (yeast)	Chromosome stability protein 20, Perinuclear array-localized protein, Ran-binding protein 1, RANBP1
3	C	Exportin-1	polymer	1023	117382.8	1	UniProt (P30822) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) In PDB	Saccharomyces cerevisiae (yeast)	CRM1, Chromosome region maintenance protein 1, Karyopherin-124
4	A	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	1	Chemie (GNP)
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	C	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	68	Chemie (HOH)

Sequence modifications

B: 62 - 201 (UniProt: P41920)

PDB	External Database	Details
Lys 98	Ala 98	conflict

C: 1 - 376 (UniProt: P30822)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

C: 414 - 1058 (UniProt: P30822)

PDB	External Database	Details
Ser 539	Thr 539	engineered mutation
Cys 1022	Tyr 1022	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	158217.7
Non-Polymers*	Number of molecules	3
	Total formula weight	582.0
All*	Total formula weight	158799.6

*Water molecules are not included.